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Prepublished online as a Blood First Edition Paper on April 24, 2003; DOI 10.1182/blood-2002-07-2206. This Article Full Text (PDF) All Versions of this Article: 2002-07-2206v1 102/5/1745    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Crawford, K. Articles by Alper, C. A Search for Related Content PubMed PubMed Citation Articles by Crawford, K. Articles by Alper, C. A Social Bookmarking                   What's this? Submitted July 26, 2002 Accepted March 3, 2003 CD2 engagement induces dendritic cell activation: implications for immune surveillance and T-cell activation Keith Crawford, Aleksandra Stark, Betsy Kitchens, Kerry Sternheim, Vassilios Pantazopoulos, Ellen Triantafellow, Zhigang Wang, Beldav Vasir, Charles E Larsen, Dana Gabuzda, Ellis Reinherz, and Chester A Alper* The Center for Blood Research, Boston, MA, USA; Department of Pediatics, Harvard Medical School, Boston, MA, USA Departments of Cancer Immunology & AIDS, Dana -Farber Cancer Institute, Boston, MA, USA Department of Adult Oncology, Dana -Farber Cancer Institute, Boston, MA, USA Department of Pathology, Harvard Medical School, Boston, MA, USA Department of Neurology, Harvard Medical School, Boston, MA, USA Department of Medicine, Harvard Medical School, Boston, MA, USA Department of Pediatics, Harvard Medical School, Boston, MA, USA * Corresponding author; email: alper{at}cbr.med.harvard.edu. We have shown previously that primary dendritic cells and monocytes express equal levels of CD14 but are distinguishable by the presence of CD2 on dendritic cells. CD2 is known to mediate the activation of T and NK cells through its interaction with CD58. CD2 epitopes recognized by anti-T111, -T112 and -T113 mAbs are present on dendritic cells. Here we show that CD2 engagement significantly increases class II, costimulatory (CD40, CD80, CD86), adhesion (CD54, CD58), and CCR7 molecule expression on primary dendritic cells. Conversely, minimal or no change in the expression of the above antigens occurs on monocyte-derived dendritic cells, since these molecules are already maximally expressed. However, both kinds of dendritic cells release IL-1 and IL-12 after CD2 engagement. Lastly, interference with dendritic cell CD2-T cell CD58 engagement decreases naive CD4+CD45RA+ T cell proliferation. Collectively, our results suggest another role of the CD2-CD58 pathway that allows nonimmune and immune cells to interact directly with dendrictic cells and initiate innate and adaptive immune responses. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: H. W. Lim and C. H. Kim Loss of IL-7 Receptor {alpha} on CD4+ T Cells Defines Terminally Differentiated B Cell-Helping Effector T Cells in a B Cell-Rich Lymphoid Tissue J. Immunol., December 1, 2007; 179(11): 7448 - 7456. [Abstract] [Full Text] [PDF] T. Di Pucchio, L. Pilla, I. Capone, M. Ferrantini, E. Montefiore, F. Urbani, R. Patuzzo, E. Pennacchioli, M. Santinami, A. Cova, et al. Immunization of Stage IV Melanoma Patients with Melan-A/MART-1 and gp100 Peptides plus IFN-{alpha} Results in the Activation of Specific CD8+ T Cells and Monocyte/Dendritic Cell Precursors. Cancer Res., May 1, 2006; 66(9): 4943 - 4951. [Abstract] [Full Text] [PDF] A. D. Narayan, J. L. Chase, R. L. Lewis, X. Tian, D. S. Kaufman, J. A. Thomson, and E. D. Zanjani Human embryonic stem cell-derived hematopoietic cells are capable of engrafting primary as well as secondary fetal sheep recipients Blood, March 1, 2006; 107(5): 2180 - 2183. [Abstract] [Full Text] [PDF] B. Vasir, D. Avigan, Z. Wu, K. Crawford, S. Turnquist, J. Ren, and D. Kufe Dendritic Cells Induce MUC1 Expression and Polarization on Human T Cells by an IL-7-Dependent Mechanism J. Immunol., February 15, 2005; 174(4): 2376 - 2386. [Abstract] [Full Text] [PDF]

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