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Proteomic identification of altered apolipoprotein patterns in pulmonary hypertension and vasculopathy of sickle cell disease
Blood Yuditskaya et al.
10.1182/blood-2008-03-142604
Supplemental materials for: Yuditskaya et al
Files in this Data Supplement:
- Document 1. Supplemental materials and methods (PDF, 71.6 KB)
- Table S1. Optimized protocol for SELDI-TOF analysis of fractionated sickle cell plasma in EDTA or heparin (PDF, 15.3 KB)
- Figure S1. Flow chart of experimental design (JPG, 92.3 KB)
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- Figure S2. Diagram of plasma specimen processing (JPG, 59.1 KB)
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Chart indicates flow of processing of patient plasma specimens fractionated by ion exchange chromatography with pH and organic solvent elution, and application to indicated affinity matrices, in duplicate SELDI-TOF runs, each read at a laser intensity optimized for low and high m/z peaks, and the resulting spectra analyzed on CiphergenExpress software to detect differences between spectra from SCD patients with PAH and those without PAH.
- Figure S3. Representative spectra and protein coverage of plasminogen precursor identified by LC-MS/MS (JPG, 127 KB)
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- Figure S4. Representative spectra and protein coverage of apolipoprotein A-I identified by LC-MS/MS (JPG, 104 KB)
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- Figure S5. Representative spectra and protein coverage of apolipoprotein A-II identified by LC-MS/MS (JPG, 99.2 KB)
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- Figure S6. Representative spectra and protein coverage Serum Amyloid A4 identified by LC-MS/MS (JPG, 103 KB)
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