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Blood, Vol. 108, Issue 4, 1381-1387, August 15, 2006
Detection of serum hepcidin in renal failure and inflammation by using ProteinChip System
Blood Tomosugi et al.
108: 1381
Supplemental materials for: Naohisa et al
Files in this Data Supplement:
- Figure S1 (PDF, 56.8 KB) -
A: On-chip optimization of buffer pH for the retention of the 2789 m/z peptide on the CM 10 chip array. The peptide remains bound to the chip at pH 10. Arrows indicate the 2192 and 2789 m/z peptide peaks.
B: On-chip optimization of NaCl concentration for desorption of the 2789 m/z peptide on the CM 10 chip array. The peptide was eluted by 500 mM NaCl. Arrow indicates the 2789 m/z peptide peak.
C: On-chip monitoring of the elution from the CM ceramic hyper DF spin column. The target peptide was eluted in 500 mM NaCl. Arrow indicates the 2789 m/z peptide peak.
D: Characterization of the purified serum peptide at 2789 m/z. The 2789 m/z peptide in the purified fraction (a) gained 8 Da after reduction by DTT (b). Due to further treatment with iodoacetamide, the peptide gained another 456 Da (c).
E: CID tandem MS spectrum of the purified peptide detected at 2789 m/z; the peptide was found to have the following sequence based on the database search: DTHFPICIFCCGCCHRSKCGMCCKT, and was unambiguously identified as hepcidin-25.
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