|
|
 Previous Article | Table of Contents | Next Article 
Blood, Vol. 96 No. 2 (July 15), 2000:
pp. 498-505
Connexin-43 gap junctions are involved in multiconnexin-expressing
stromal support of hemopoietic progenitors and stem cells
Jose A. Cancelas,
Wendy L. M. Koevoet,
Alexandra
E. de Koning,
Angelique E. M. Mayen,
Elwin J. C. Rombouts, and
Rob E. Ploemacher
From the Department of Hematology, Faculty of Medicine, Erasmus
University of Rotterdam, The Netherlands.
Gap junctions (GJs) provide for a unique system of intercellular
communication (IC) allowing rapid transport of small molecules from
cell to cell. GJs are formed by a large family of proteins named
connexins (Cxs). Cx43 has been considered as the predominantly expressed Cx by hematopoietic-supporting stroma. To investigate the
role of the Cx family in hemopoiesis, we analyzed the expression of 11 different Cx species in different stromal cell lines derived from
murine bone marrow (BM) or fetal liver (FL). We found that up to 5 Cxs
are expressed in FL stromal cells (Cx43, Cx45, Cx30.3, Cx31, and
Cx31.1), whereas only Cx43, Cx45, and Cx31 were clearly detectable in
BM stromal cells. In vivo, the Cx43-deficient 14.5- to 15-day FL
cobblestone area-forming cells (CAFC)-week 1-4 and colony-forming unit
contents were 26%-38% and 39%-47% lower than in their wild-type
counterparts, respectively. The reintroduction of the Cx43 gene into
Cx43-deficient FL stromal cells was able to restore their diminished IC
to the level of the wild-type FL stromal cells. In addition, these
Cx43-reintroduced stromal cells showed an increased support ability
(3.7-fold) for CAFC-week 1 in normal mouse BM and 5-fold higher
supportive ability for CAFC-week 4 in 5-fluorouracil-treated BM cells
as compared with Cx43-deficient FL stromal cells. These findings
suggest that stromal Cx43-mediated IC, although not responsible for all
GJ-mediated IC of stromal cells, plays a role in the supportive ability
for hemopoietic progenitors and stem cells.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
E. J. Paredes-Gamero, C. M. M. P. Leon, R. Borojevic, M. E. M. Oshiro, and A. T. Ferreira
Changes in Intracellular Ca2+ Levels Induced by Cytokines and P2 Agonists Differentially Modulate Proliferation or Commitment with Macrophage Differentiation in Murine Hematopoietic Cells
J. Biol. Chem.,
November 14, 2008;
283(46):
31909 - 31919.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. M.-H. Yeung, U. Schlotzer-Schrehardt, B. Kulkarni, N. L. Tint, A. Hopkinson, and H. S. Dua
Limbal Epithelial Crypt: A Model for Corneal Epithelial Maintenance and Novel Limbal Regional Variations
Arch Ophthalmol,
May 1, 2008;
126(5):
665 - 669.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Rota, J. Kajstura, T. Hosoda, C. Bearzi, S. Vitale, G. Esposito, G. Iaffaldano, M. E. Padin-Iruegas, A. Gonzalez, R. Rizzi, et al.
Bone marrow cells adopt the cardiomyogenic fate in vivo
PNAS,
November 6, 2007;
104(45):
17783 - 17788.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Romieu-Mourez, M. Francois, M.-N. Boivin, J. Stagg, and J. Galipeau
Regulation of MHC Class II Expression and Antigen Processing in Murine and Human Mesenchymal Stromal Cells by IFN-{gamma}, TGF-beta, and Cell Density
J. Immunol.,
August 1, 2007;
179(3):
1549 - 1558.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Hirabayashi, B.-I. Yoon, I. Tsuboi, Y. Huo, Y. Kodama, J. Kanno, T. Ott, J. E. Trosko, and T. Inoue
Protective Role of Connexin 32 in Steady-State Hematopoiesis, Regeneration State, and Leukemogenesis
Experimental Biology and Medicine,
May 1, 2007;
232(5):
700 - 712.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. A Shanmuganathan, T. Foster, B. B Kulkarni, A. Hopkinson, T. Gray, D. G Powe, J. Lowe, and H. S Dua
Morphological characteristics of the limbal epithelial crypt
Br. J. Ophthalmol.,
April 1, 2007;
91(4):
514 - 519.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Ye and E. Eskin
Discovering tightly regulated and differentially expressed gene sets in whole genome expression data
Bioinformatics,
January 15, 2007;
23(2):
e84 - e90.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. J. B. Francis and C. W. Lo
Primordial germ cell deficiency in the connexin 43 knockout mouse arises from apoptosis associated with abnormal p53 activation
Development,
September 1, 2006;
133(17):
3451 - 3460.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Urbanek, D. Cesselli, M. Rota, A. Nascimbene, A. De Angelis, T. Hosoda, C. Bearzi, A. Boni, R. Bolli, J. Kajstura, et al.
From the Cover: Stem cell niches in the adult mouse heart
PNAS,
June 13, 2006;
103(24):
9226 - 9231.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. M. Flenniken, L. R. Osborne, N. Anderson, N. Ciliberti, C. Fleming, J. E. I. Gittens, X.-Q. Gong, L. B. Kelsey, C. Lounsbury, L. Moreno, et al.
A Gja1 missense mutation in a mouse model of oculodentodigital dysplasia
Development,
October 1, 2005;
132(19):
4375 - 4386.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. W Wong, T. Christen, and B. R Kwak
Connexins in leukocytes: shuttling messages?
Cardiovasc Res,
May 1, 2004;
62(2):
357 - 367.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Rosendaal and C. Jopling
Hematopoietic capacity of connexin43 wild-type and knock-out fetal liver cells not different on wild-type stroma
Blood,
April 15, 2003;
101(8):
2996 - 2998.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Oviedo-Orta and W. H. Evans
Gap junctions and connexins: potential contributors to the immunological synapse
J. Leukoc. Biol.,
October 1, 2002;
72(4):
636 - 642.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Montecino-Rodriguez and K. Dorshkind
Regulation of hematopoiesis by gap junction-mediated intercellular communication
J. Leukoc. Biol.,
September 1, 2001;
70(3):
341 - 347.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
