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Analysis of the survival of mature human eosinophils: interleukin-5
prevents apoptosis in mature human eosinophils
Y Yamaguchi, T Suda, S Ohta, K Tominaga, Y Miura and T Kasahara
Department of Medical Biology and Parasitology, Jichi Medical School,
Tochigi-ken, Japan.
We and other groups have previously shown that interleukin-5 (IL-5)
maintained the viability of mature eosinophils in an in vitro liquid
culture system. Mature eosinophils did not proliferate but their survival
was maintained in the presence of IL-5. Using this culture system, we
investigated the mechanism of IL-5-mediated survival. In the absence of
human IL-5 (hIL-5) mature eosinophils succumbed after 4 days, while in the
presence of hIL-5 they survived up to 10 days. When DNA extracts of
cultured eosinophils were analyzed on an agar gel electrophoresis, marked
DNA fragmentation was observed in the absence of hIL-5, while no
significant DNA fragmentation was observed in the culture with hIL-5 for 48
hours. The DNA fragmentation appeared as early as 6 to 12 hours after hIL-5
deprivation. Concomitantly, IL-5 stimulated total RNA and protein
synthesis, but did not induce DNA synthesis in mature eosinophils. Because
cycloheximide or actinomycin D impeded the protection of apoptosis by
hIL-5, some new RNA and protein synthesis appeared to be required in this
phenomena. These findings indicate that IL-5 maintains survival of mature
eosinophils with induction of new RNA and protein synthesis, thus leading
to the inhibition of apoptosis.
Volume 78,
Issue 10,
pp. 2542-2547,
11/15/1991
Copyright © 1991 by The American Society of Hematology
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