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Basic fibroblast growth factor stimulates myelopoiesis in long-term human
bone marrow cultures
EL Wilson, DB Rifkin, F Kelly, MJ Hannocks and JL Gabrilove
Department of Cell Biology, New York University Medical Center, NY 10016.
We previously showed that basic fibroblast growth factor (bFGF) is a potent
mitogen for human bone marrow (BM) stromal cells and significantly delays
their senescence. In the present study, we demonstrated that low
concentrations of bFGF (0.2 to 2 ng/mL) enhance myelopoiesis in long-term
human BM culture. Addition of bFGF to long- term BM cultures resulted in an
increase in (a) the number of nonadherent cells (sixfold), particularly
those of the neutrophil granulocyte series; (b) the number of nonadherent
granulocyte colony- stimulating factor (G-CSF)- and granulocyte-macrophage
colony- stimulating factor (GM-CSF)-responsive progenitor cells; (c) the
number of adherent foci of hematopoietic cells (10-fold); and (d) the
number of progenitor cells in the adherent stromal cell layer. These
effects were not noted with higher concentrations of bFGF (20 ng/mL). Thus,
low concentrations of bFGF effectively augment myelopoiesis in human long-
term BM cultures, and bFGF may therefore be a regulator of the
hematopoietic system in vitro and in vivo.
Volume 77,
Issue 5,
pp. 954-960,
03/01/1991
Copyright © 1991 by The American Society of Hematology
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