Analysis of junctional diversity in the preferential V delta 1-J delta 1
rearrangement of fresh T-acute lymphoblastic leukemia cells by in vitro
gene amplification and direct sequencing
E Macintyre, L d'Auriol, F Amesland, P Loiseau, Z Chen, L Boumsell, F Galibert and F Sigaux
Laboratoire d'Hematologie Moleculaire, Hopital Saint Louis, Paris, France.
To define the junctional diversity of T-cell antigen receptor delta gene
rearrangements in fresh T-acute lymphoblastic cells and to correlate cell
phenotype with the coding potential of rearrangements, we determined the
junctional nucleotide sequences of 13 T-cell antigen receptor delta gene
rearrangements involving the preferentially rearranged V (V delta 1) and J
(J delta 1) segments using in vitro gene amplification and direct
sequencing. We showed that, as in gamma delta+ cell lines, extensive
junctional diversity exists in these clones and that this diversity is due
both to random nucleotide deletions/additions and to the use of at least
two D delta segments. We also showed that a high percentage of these
rearrangements are potentially translatable (7:13) and that such functional
rearrangements occur in both surface CD3+ and CD3- cells. Comparison of
alpha beta versus gamma delta surface expression demonstrates that all CD3+
T acute lymphoblastic leukemias with a functional V delta 1-J delta 1
rearrangement express a surface gamma delta receptor and are recognized by
the anti-delta monoclonal antibody delta TCS1, whereas a control CD3+ gamma
delta+ leukemic case that had not undergone V delta 1 rearrangement was
delta TCS1-. In addition, expression of this monoclonal antibody is not
restricted by V gamma or C gamma usage or by the covalent or noncovalent
link between gamma and delta chains.
Volume 74,
Issue 6,
pp. 2053-2061,
11/01/1989
Copyright © 1989 by The American Society of Hematology
