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Osmotic gradient ektacytometry: comprehensive characterization of red cell
volume and surface maintenance
MR Clark, N Mohandas and SB Shohet
Whole cell deformability of red cells was measured as a continuous function
of suspending medium osmolality using the ektacytometer, a
laser-diffraction viscometer. Study of normal cells in which water content
and membrane surface area had been selectively modified showed that this
technique can detect changes in these properties with high sensitivity. The
osmotic deformability profiles obtained from this assay provide information
about cell water content, surface area, and the heterogeneity in these
cellular properties, information that by conventional methods would require
several different types of measurements. Application of this approach to a
variety of pathologic blood samples showed that various hematologic
disorders can be characterized by the shape of this profile and the
position of specific features of the profile along the osmolality axis.
Measurement of osmotic deformability profiles thus provides a convenient
and comprehensive means of identifying abnormalities either in red cell
water content or surface area.
Volume 61,
Issue 5,
pp. 899-910,
05/01/1983
Copyright © 1983 by The American Society of Hematology
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