Oxidative metabolic responses of rabbit pulmonary alveolar macrophages
LA Boxer, G Ismail, JM Allen and RL Baehner
During phagocytosis of opsonized lipopolysaccharide-coated paraffin oil
droplets, rabbit alveolar macrophages reduced nitroblue tetrazolium, which
effect was in part inhibitable with the use of superoxide dismutase.
Exposure of cytochalasin-B-treated rabbit alveolar macrophages to opsonized
zymosan led to the generation of superoxide, as quantitated by
ferricytochrome C reduction. It was found that nitroblue tetrazolium in the
presence of ferricytochrome C could in turn serve as scavenger of
superoxide during stimulation of cytochalasin-B-treated rabbit alveolar
macrophages. Following challenge with either opsonized zymosan or the
membrane perturbant digitonin, rabbit alveolar macrophages released
hydrogen peroxide into the extracellular medium. Employment of the surface
membrane stimulant phorbol myristrate acetate led to activation of the
hexose monophosphate shunt, which activity could be further enhanced in the
presence of superoxide dismutase or attenuated in the presence of catalase.
These studies demonstrate that rabbit alveolar macrophages release
superoxide and hydrogen peroxide during surface membrane perturbation. In
turn, hydrogen peroxide generation can stimulate the hexose monophosphate
shunt.
Volume 53,
Issue 3,
pp. 486-491,
03/01/1979
Copyright © 1979 by The American Society of Hematology
