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Oxidative metabolism of the human eosinophil
LR DeChatelet, PS Shirley, LC McPhail, CC Huntley, HB Muss and DA Bass
We have compared the oxidative metabolism of human eosinophils (80%-90%
purity) to that of neutrophils. Hexose monophosphate (HMP) shunt activity
of eosinophils was higher than that of neutrophils under either resting or
phagocytizing conditions. Eosinophil HMP shunt activity also was stimulated
by phorbol myristate acetate, a membrane- active agent. Eosinophils showed
a marked incorporation of 125I into trichloroacetic acid-insoluble material
under resting conditions, which increased markedly during phagocytosis.
Eosinophils likewise showed a greater reduction of nitroblue tetrazolium
dye during phagocytosis than did neutrophils. Measurement of other
parameters of oxidative metabolism indicated that eosinophils generated
superoxide anion following phagocytosis and also elicited a burst of
chemiluminescence similar to that observed during phagocytosis by
neutrophils. Measurement of NADPH oxidase activity demonstrated that this
enzyme was 3-6 times more active in fractions isolated from eosinophils
than in corresponding fractions isolated from neutrophils; this was
observed over a range of substrate concentrations. The eosinophil enzyme
sedimented differently than the neutrophil enzyme with differential
centrifugation; neither showed sedimentation characteristics of peroxidase.
These data indicate that eosinophils possess a similar, although in some
ways more potent, oxidative burst than neutrophils and are consistent with
a role for NADPH oxidase in the initiation of that burst.
Volume 50,
Issue 3,
pp. 525-535,
09/01/1977
Copyright © 1977 by The American Society of Hematology
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