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 Blood, 15 June 2006, Vol. 107, No. 12, pp. 4711-4713.
Prepublished online as a Blood First Edition Paper on February 21, 2006; DOI 10.1182/blood-2006-01-0028.

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Submitted January 5, 2006
Accepted February 11, 2006

False-positive detection of recombinant human erythropoietin in urine following strenuous physical exercise

Monique Beullens, Joris R Delanghe, and Mathieu Bollen*

Division of Biochemistry, Department of Molecular Cell Biology, Faculty of Medicine, Catholic University of Leuven, Leuven, Belgium
Department of Clinical Chemistry, University Hospital, Gent, Belgium

* Corresponding author; email: Mathieu.Bollen{at}med.kuleuven.be.

Erythropoietin (Epo) is a glycoprotein hormone that promotes the production of red blood cells. Recombinant human Epo (rhEpo) is illicitly used to improve performance in endurance sports. Doping in sports is discouraged by the screening of athletes for rhEPO in urine. The adopted test is based on a combination of isoelectric focusing and double immunoblotting, and distinguishes between endogenous and recombinant human Epo. We show here that this widely-used test can occasionally lead to the false-positive detection of rhEpo (epoetin-{beta}) in post-exercise, protein-rich urine, probably because the adopted monoclonal anti-Epo antibodies are not monospecific.


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Related Letters in Blood Online:

False-positive Epo test concerns unfounded
Don Catlin, Gary Green, Michael Sekera, Paul Scott, and Borislav Starcevic
Blood 2006 108: 1778. [Full Text] [PDF]

No doubt about the validity of the urine test for detection of recombinant human erythropoietin
Françoise Lasne, Monique Beullens, Joris R. Delanghe, and Mathieu Bollen
Blood 2006 108: 1778-1780. [Full Text] [PDF]



This article has been cited by other articles:


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 BloodHome page
D. Catlin, G. Green, M. Sekera, P. Scott, and B. Starcevic
False-positive Epo test concerns unfounded.
Blood, September 1, 2006; 108(5): 1778 - 1778.
[Full Text] [PDF]

Home page
 BloodHome page
F. Lasne, M. Beullens, J. R. Delanghe, and M. Bollen
No doubt about the validity of the urine test for detection of recombinant human erythropoietin.
Blood, September 1, 2006; 108(5): 1778 - 1780.
[Full Text] [PDF]


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