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Blood, 15 August 2006, Vol. 108, No. 4, pp. 1381-1387. Prepublished online as a Blood First Edition Paper on April 18, 2006; DOI 10.1182/blood-2005-10-4043. This Article Full Text (PDF) Supplemental Figure All Versions of this Article: 2005-10-4043v1 108/4/1381    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Tomosugi, N. Articles by Ishikawa, I. Search for Related Content PubMed PubMed Citation Articles by Tomosugi, N. Articles by Ishikawa, I. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted October 13, 2005 Accepted March 23, 2006 Detection of serum hepcidin in renal failure and inflammation by using ProteinChip System® Naohisa Tomosugi*, Hiroshi Kawabata, Rumi Wakatabe, Masato Higuchi, Hideki Yamaya, Hisanori Umehara, and Isao Ishikawa Division of Nephrology, Department of Internal Medicine, Kanazawa Medical University, Ishikawa, Japan Department of Hematology and Oncology, Graduate School of Medicine, Kyoto University, Kyoto, Japan Ciphergen Biosystems, Biomarker Discovery Center, Yokohama, Japan Department of Toxin, Chugai Pharma Co.LTD, Shizuoka, Japan * Corresponding author; email: tomosugi{at}kanazawa-med.ac.jp. Hepcidin, a key regulator of iron metabolism, is expressed in the liver, distributed in blood, and excreted in urine. However, to date, no reliable and practical method for measuring the bioactive form of hepcidin in serum has been developed. Here, we employed surface-enhanced laser desorption ionization time of flight mass spectrometry (SELDI-TOF MS) to analyze the distinctive serum proteomic patterns of hemodialysis patients. In the range of 1000 to 15,000 m/z, we found three peptides at 2192, 2789, and 2851 m/z that showed a significant correlation with the serum ferritin levels. The molecular sizes of peptides at 2192 and 2789 m/z matched with the reported sizes of hepcidin-20 and -25, respectively, and the serum peptide at 2789 m/z was identified as hepcidin-25 by collision-induced dissociation tandem MS. By employing SELDI-TOF MS, we developed a semiquantitative assay for hepcidin-25. In this assay, the level of serum hepcidin-25 correlated well with those of serum ferritin and serum interleukin-6. Hepcidin-25 was found to accumulate in the serum of hemodialysis patients; this could contribute to the pathogenesis of renal anemia by decreasing the available iron for hematopoiesis. Thus, SELDI-TOF MS would be a clinically useful tool to detect and semiquantify bioactive hepcidin in serum. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: J. Kanda, C. Mizumoto, H. Kawabata, H. Tsuchida, N. Tomosugi, K. Matsuo, and T. Uchiyama Serum hepcidin level and erythropoietic activity after hematopoietic stem cell transplantation Haematologica, October 1, 2008; 93(10): 1550 - 1554. [Abstract] [Full Text] [PDF] D. W. Swinkels and J. F. M. Wetzels Hepcidin: a new tool in the management of anaemia in patients with chronic kidney disease? Nephrol. Dial. Transplant., May 21, 2008; (2008) gfn267v1. [Full Text] [PDF] E. H.J.M. Kemna, H. Tjalsma, H. L. Willems, and D. W. Swinkels Hepcidin: from discovery to differential diagnosis Haematologica, January 1, 2008; 93(1): 90 - 97. [Abstract] [Full Text] [PDF] A. T. Murphy, D. R. Witcher, P. Luan, and V. J. Wroblewski Quantitation of hepcidin from human and mouse serum using liquid chromatography tandem mass spectrometry Blood, August 1, 2007; 110(3): 1048 - 1054. [Abstract] [Full Text] [PDF] H. Kawabata, N. Tomosugi, J. Kanda, Y. Tanaka, K. Yoshizaki, and T. Uchiyama Anti-interleukin 6 receptor antibody tocilizumab reduces the level of serum hepcidin in patients with multicentric Castleman's disease Haematologica, June 1, 2007; 92(6): 857 - 858. [Abstract] [Full Text] [PDF] E. H.J.M. Kemna, H. Tjalsma, V. N. Podust, and D. W. Swinkels Mass Spectrometry-Based Hepcidin Measurements in Serum and Urine: Analytical Aspects and Clinical Implications Clin. Chem., April 1, 2007; 53(4): 620 - 628. [Abstract] [Full Text] [PDF] T. Ganz Molecular Control of Iron Transport J. Am. Soc. Nephrol., February 1, 2007; 18(2): 394 - 400. [Abstract] [Full Text] [PDF] J. Himmelfarb Iron Regulation J. Am. Soc. Nephrol., February 1, 2007; 18(2): 379 - 381. [Full Text] [PDF] T. Ganz Hepcidin and Its Role in Regulating Systemic Iron Metabolism Hematology, January 1, 2006; 2006(1): 29 - 35. [Abstract] [Full Text] [PDF]

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