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Blood, 1 June 2004, Vol. 103, No. 11, pp. 4322-4329. Prepublished online as a Blood First Edition Paper on February 5, 2004; DOI 10.1182/blood-2003-11-4041. This Article Full Text Full Text (PDF) All Versions of this Article: 2003-11-4041v1 103/11/4322    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Montagna, D. Articles by Maccario, R. Search for Related Content PubMed PubMed Citation Articles by Montagna, D. Articles by Maccario, R. Related Collections Immunobiology Transplantation Clinical Trials and Observations Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  TRANSPLANTATION T lymphocytes of recipient origin may contribute to the recovery of specific immune response toward viruses and fungi in children undergoing cord blood transplantation Daniela Montagna, Franco Locatelli, Antonia Moretta, Daniela Lisini, Carlo Previderè, Pierangela Grignani, Piero DeStefano, Giovanna Giorgiani, Enrica Montini, Sara Pagani, Patrizia Comoli, and Rita Maccario From the Dipartimento di Scienze Pediatriche, Università di Pavia, Pavia, Italy; Oncoematologia Pediatrica, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) Policlinico San Matteo, Pavia, Italy; and Laboratorio di Ematologia Forense, Istituto di Medicina Legale, Università di Pavia, Pavia, Italy. Patients undergoing allogeneic cord blood transplantation (CBT) benefit from a low risk of graft-versus-host disease (GVHD), but there are still concerns that they be able to recover an effective immune capacity early after transplantation. We investigated the ability to develop in vitro T-lymphocyte-mediated immune response toward human cytomegalovirus and Candida albicans antigens, early and late after transplantation, in children given cord blood transplants from either an HLA-identical sibling or an unrelated donor. Proliferative capacity and frequency of antigen-specific T cells were evaluated; antigen-specific CD4+ T-cell clones were also generated and characterized for T-cell receptor repertoire diversity, cytokine phenotype, and their origin (either from donor or patient). We found that the majority of recipients can develop a specific response to viral or fungal antigens already early after transplantation. Antigen-specific T-cell clones of both donor and recipient origin contributed to the reconstitution of immune response. Antigen-specific T lymphocytes of recipient origin were detected in patients receiving a transplant from a relative, after a chemotherapy-based conditioning regimen, and who did not have GVHD. Our results document, at a clonal level, that after CBT recovery of either polyclonal or pauciclonal T-cell response toward widespread pathogens is prompt, with some patients benefiting from a contribution of recipient-derived cells. (Blood. 2004;103:4322-4329) CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: D. Lisini, M. Zecca, G. Giorgiani, D. Montagna, R. Cristantielli, M. Labirio, P. Grignani, C. Previdere, A. Di Cesare-Merlone, G. Amendola, et al. Donor/recipient mixed chimerism does not predict graft failure in children with {beta}-thalassemia given an allogeneic cord blood transplant from an HLA-identical sibling Haematologica, December 1, 2008; 93(12): 1859 - 1867. [Abstract] [Full Text] [PDF] J. Smythe, S. Armitage, D. McDonald, D. Pamphilon, M. Guttridge, J. Brown, A. Green, C. Brown, R. M. Warwick, A. Lankester, et al. Directed Sibling Cord Blood Banking for Transplantation: The 10-Year Experience in the National Blood Service in England Stem Cells, August 1, 2007; 25(8): 2087 - 2093. [Abstract] [Full Text] [PDF] D. Montagna, R. Maccario, F. Locatelli, E. Montini, S. Pagani, F. Bonetti, L. Daudt, I. Turin, D. Lisini, C. Garavaglia, et al. Emergence of antitumor cytolytic T cells is associated with maintenance of hematologic remission in children with acute myeloid leukemia Blood, December 1, 2006; 108(12): 3843 - 3850. [Abstract] [Full Text] [PDF] D. Lilleri, G. Gerna, C. Fornara, L. Lozza, R. Maccario, and F. Locatelli Prospective simultaneous quantification of human cytomegalovirus-specific CD4+ and CD8+ T-cell reconstitution in young recipients of allogeneic hematopoietic stem cell transplants Blood, August 15, 2006; 108(4): 1406 - 1412. [Abstract] [Full Text] [PDF] Y. Chalandon, S. Degermann, J. Villard, L. Arlettaz, L. Kaiser, S. Vischer, S. Walter, M. H. M. Heemskerk, R. A. W. van Lier, C. Helg, et al. Pretransplantation CMV-specific T cells protect recipients of T-cell-depleted grafts against CMV-related complications Blood, January 1, 2006; 107(1): 389 - 396. [Abstract] [Full Text] [PDF]

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